Evaluation of cellulase preparations for hydrolysis of hardwood substrates.
Identifieur interne : 003E23 ( Main/Exploration ); précédent : 003E22; suivant : 003E24Evaluation of cellulase preparations for hydrolysis of hardwood substrates.
Auteurs : Alex Berlin [Canada] ; Neil Gilkes ; Douglas Kilburn ; Vera Maximenko ; Renata Bura ; Alexander Markov ; Anton Skomarovsky ; Alexander Gusakov ; Arkady Sinitsyn ; Oleg Okunev ; Irina Solovieva ; John N. SaddlerSource :
- Applied biochemistry and biotechnology [ 0273-2289 ] ; 2006.
Descripteurs français
- KwdFr :
- Activation enzymatique (MeSH), Arbres (composition chimique), Bois (MeSH), Cellulase (classification), Cellulase (composition chimique), Cellulose (composition chimique), Cinétique (MeSH), Hydrolyse (MeSH), Modèles biologiques (MeSH), Modèles chimiques (MeSH), Simulation numérique (MeSH), Spécificité du substrat (MeSH).
- MESH :
- classification : Cellulase.
- composition chimique : Arbres, Cellulase, Cellulose.
- Activation enzymatique, Bois, Cinétique, Hydrolyse, Modèles biologiques, Modèles chimiques, Simulation numérique, Spécificité du substrat.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Cellulase, Cellulose.
- chemical , classification : Cellulase.
- chemistry : Trees.
- Computer Simulation, Enzyme Activation, Hydrolysis, Kinetics, Models, Biological, Models, Chemical, Substrate Specificity, Wood.
Abstract
Seven cellulase preparations from Penicillium and Trichoderma spp. were evaluated for their ability to hydrolyze the cellulose fraction of hardwoods (yellow poplar and red maple) pretreated by organosolv extraction, as well as model cellulosic substrates such as filter paper. There was no significant correlation among hydrolytic performance on pretreated hardwood, based on glucose release, and filter paper activity. However, performance on pretreated hardwood showed significant correlations to the levels of endogenous beta-glucosidase and xylanase activities in the cellulase preparation. Accordingly, differences in performance were reduced or eliminated following supplementation with a crude beta-glucosidase preparation containing both activities. These results complement a previous investigation using softwoods pretreated by either organosolv extraction or steam explosion. Cellulase preparations that performed best on hardwood also showed superior performance on the softwood substrates.
DOI: 10.1385/abab:130:1:528
PubMed: 16915667
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Saddler</name></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="ISSN">0273-2289</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cellulase (chemistry)</term><term>Cellulase (classification)</term><term>Cellulose (chemistry)</term><term>Computer Simulation (MeSH)</term><term>Enzyme Activation (MeSH)</term><term>Hydrolysis (MeSH)</term><term>Kinetics (MeSH)</term><term>Models, Biological (MeSH)</term><term>Models, Chemical (MeSH)</term><term>Substrate Specificity (MeSH)</term><term>Trees (chemistry)</term><term>Wood (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation enzymatique (MeSH)</term><term>Arbres (composition chimique)</term><term>Bois (MeSH)</term><term>Cellulase (classification)</term><term>Cellulase (composition chimique)</term><term>Cellulose (composition chimique)</term><term>Cinétique (MeSH)</term><term>Hydrolyse (MeSH)</term><term>Modèles biologiques (MeSH)</term><term>Modèles chimiques (MeSH)</term><term>Simulation numérique (MeSH)</term><term>Spécificité du substrat (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulase</term><term>Cellulose</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Cellulase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Trees</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Cellulase</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Arbres</term><term>Cellulase</term><term>Cellulose</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Computer Simulation</term><term>Enzyme Activation</term><term>Hydrolysis</term><term>Kinetics</term><term>Models, Biological</term><term>Models, Chemical</term><term>Substrate Specificity</term><term>Wood</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation enzymatique</term><term>Bois</term><term>Cinétique</term><term>Hydrolyse</term><term>Modèles biologiques</term><term>Modèles chimiques</term><term>Simulation numérique</term><term>Spécificité du substrat</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Seven cellulase preparations from Penicillium and Trichoderma spp. were evaluated for their ability to hydrolyze the cellulose fraction of hardwoods (yellow poplar and red maple) pretreated by organosolv extraction, as well as model cellulosic substrates such as filter paper. There was no significant correlation among hydrolytic performance on pretreated hardwood, based on glucose release, and filter paper activity. However, performance on pretreated hardwood showed significant correlations to the levels of endogenous beta-glucosidase and xylanase activities in the cellulase preparation. Accordingly, differences in performance were reduced or eliminated following supplementation with a crude beta-glucosidase preparation containing both activities. These results complement a previous investigation using softwoods pretreated by either organosolv extraction or steam explosion. Cellulase preparations that performed best on hardwood also showed superior performance on the softwood substrates.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16915667</PMID><DateCompleted><Year>2006</Year><Month>09</Month><Day>15</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>11</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0273-2289</ISSN><JournalIssue CitedMedium="Print"><Volume>129-132</Volume><PubDate><Year>2006</Year><Season>Spring</Season></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl Biochem Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Evaluation of cellulase preparations for hydrolysis of hardwood substrates.</ArticleTitle><Pagination><MedlinePgn>528-45</MedlinePgn></Pagination><Abstract><AbstractText>Seven cellulase preparations from Penicillium and Trichoderma spp. were evaluated for their ability to hydrolyze the cellulose fraction of hardwoods (yellow poplar and red maple) pretreated by organosolv extraction, as well as model cellulosic substrates such as filter paper. There was no significant correlation among hydrolytic performance on pretreated hardwood, based on glucose release, and filter paper activity. However, performance on pretreated hardwood showed significant correlations to the levels of endogenous beta-glucosidase and xylanase activities in the cellulase preparation. Accordingly, differences in performance were reduced or eliminated following supplementation with a crude beta-glucosidase preparation containing both activities. These results complement a previous investigation using softwoods pretreated by either organosolv extraction or steam explosion. Cellulase preparations that performed best on hardwood also showed superior performance on the softwood substrates.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Berlin</LastName><ForeName>Alex</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Forest Products Biotechnology, Faculty of Forestry, The University of British Columbia, Vancouver, BC V6T 1Z4, Canada. alex.berlin@ubc.ca</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gilkes</LastName><ForeName>Neil</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Kilburn</LastName><ForeName>Douglas</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Maximenko</LastName><ForeName>Vera</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Bura</LastName><ForeName>Renata</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Markov</LastName><ForeName>Alexander</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Skomarovsky</LastName><ForeName>Anton</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Gusakov</LastName><ForeName>Alexander</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Sinitsyn</LastName><ForeName>Arkady</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Okunev</LastName><ForeName>Oleg</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Solovieva</LastName><ForeName>Irina</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Saddler</LastName><ForeName>John N</ForeName><Initials>JN</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Biochem Biotechnol</MedlineTA><NlmUniqueID>8208561</NlmUniqueID><ISSNLinking>0273-2289</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003198" MajorTopicYN="N">Computer Simulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004789" MajorTopicYN="N">Enzyme Activation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008954" MajorTopicYN="Y">Models, Biological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008956" MajorTopicYN="Y">Models, Chemical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="Y">Wood</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>8</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>9</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>8</Month><Day>19</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16915667</ArticleId><ArticleId IdType="doi">10.1385/abab:130:1:528</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Bura, Renata" sort="Bura, Renata" uniqKey="Bura R" first="Renata" last="Bura">Renata Bura</name><name sortKey="Gilkes, Neil" sort="Gilkes, Neil" uniqKey="Gilkes N" first="Neil" last="Gilkes">Neil Gilkes</name><name sortKey="Gusakov, Alexander" sort="Gusakov, Alexander" uniqKey="Gusakov A" first="Alexander" last="Gusakov">Alexander Gusakov</name><name sortKey="Kilburn, Douglas" sort="Kilburn, Douglas" uniqKey="Kilburn D" first="Douglas" last="Kilburn">Douglas Kilburn</name><name sortKey="Markov, Alexander" sort="Markov, Alexander" uniqKey="Markov A" first="Alexander" last="Markov">Alexander Markov</name><name sortKey="Maximenko, Vera" sort="Maximenko, Vera" uniqKey="Maximenko V" first="Vera" last="Maximenko">Vera Maximenko</name><name sortKey="Okunev, Oleg" sort="Okunev, Oleg" uniqKey="Okunev O" first="Oleg" last="Okunev">Oleg Okunev</name><name sortKey="Saddler, John N" sort="Saddler, John N" uniqKey="Saddler J" first="John N" last="Saddler">John N. Saddler</name><name sortKey="Sinitsyn, Arkady" sort="Sinitsyn, Arkady" uniqKey="Sinitsyn A" first="Arkady" last="Sinitsyn">Arkady Sinitsyn</name><name sortKey="Skomarovsky, Anton" sort="Skomarovsky, Anton" uniqKey="Skomarovsky A" first="Anton" last="Skomarovsky">Anton Skomarovsky</name><name sortKey="Solovieva, Irina" sort="Solovieva, Irina" uniqKey="Solovieva I" first="Irina" last="Solovieva">Irina Solovieva</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Berlin, Alex" sort="Berlin, Alex" uniqKey="Berlin A" first="Alex" last="Berlin">Alex Berlin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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